› 5wwq

X-ray diffraction
2.82Å resolution

Crystal structure of human NSun6

Released:
DOI: 10.2210/pdb5wwq/pdb
PDB entry 5wwq coloured by chain, front view.
PDB entry 5wwq coloured by chain, side view.
PDB entry 5wwq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for substrate binding and catalytic mechanism of a human RNA:m5C methyltransferase NSun6.
Liu RJ, Long T, Li J, Li H, Wang ED
Nucleic Acids Res 45 6684-6697 (2017)
PMID: 28531330

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186236 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (cytosine(72)-C(5))-methyltransferase NSUN6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 477 amino acids
Theoretical weight: 52.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: Q8TEA1 (Residues: 1-469; Coverage: 100%)
Gene names: NOPD1, NSUN6
Sequence domains: 16S rRNA methyltransferase RsmB/F

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 66.965Å b: 71.877Å c: 219.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.267
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

Quick links

Citations

20 review citations

Eukaryotic 5-methylcytosine (m⁵C) RNA Methyltransferases: Mechanisms, Cellular Functions, and Links to Disease.
Bohnsack et al. (2019)
19 more

3 mentions without citation

Structural basis for substrate binding and catalytic mechanism of a human RNA:m5C methyltransferase NSun6.
Liu et al. (2017)
2 more