› 5wwr

X-ray diffraction
3.1Å resolution

Crystal structure of human NSun6/tRNA/SFG

Released:
DOI: 10.2210/pdb5wwr/pdb
PDB entry 5wwr coloured by chain, front view.
PDB entry 5wwr coloured by chain, side view.
PDB entry 5wwr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for substrate binding and catalytic mechanism of a human RNA:m5C methyltransferase NSun6.
Liu RJ, Long T, Li J, Li H, Wang ED
Nucleic Acids Res 45 6684-6697 (2017)
PMID: 28531330

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186237 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
tRNA (cytosine(72)-C(5))-methyltransferase NSUN6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 477 amino acids
Theoretical weight: 52.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: Q8TEA1 (Residues: 1-469; Coverage: 100%)
Gene names: NOPD1, NSUN6
Sequence domains: 16S rRNA methyltransferase RsmB/F
tRNA Chains: C, D
Molecule details ›
Chains: C, D
Length: 75 nucleotides
Theoretical weight: 24.17 KDa
Source organism: Homo sapiens
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

1 bound ligand:
Ligand SFG 2 x SFG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 206.908Å b: 170.478Å c: 76.999Å
α: 90° β: 108.55° γ: 90°
R-values:
R R work R free
0.197 0.195 0.235
Expression systems:
  • Escherichia coli-Pichia pastoris shuttle vector pPpARG4
  • Not provided

Quick links

Citations

20 review citations

Eukaryotic 5-methylcytosine (m⁵C) RNA Methyltransferases: Mechanisms, Cellular Functions, and Links to Disease.
Bohnsack et al. (2019)
19 more

3 mentions without citation

Structural basis for substrate binding and catalytic mechanism of a human RNA:m5C methyltransferase NSun6.
Liu et al. (2017)
2 more