5wwt

X-ray diffraction
3.2Å resolution

Crystal structure of human NSun6/tRNA

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-186237 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
tRNA (cytosine(72)-C(5))-methyltransferase NSUN6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 477 amino acids
Theoretical weight: 52.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: Q8TEA1 (Residues: 1-469; Coverage: 100%)
Gene names: NOPD1, NSUN6
Sequence domains: 16S rRNA methyltransferase RsmB/F
tRNA Chains: C, D
Molecule details ›
Chains: C, D
Length: 75 nucleotides
Theoretical weight: 24.17 KDa
Source organism: Homo sapiens
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 205.586Å b: 170.117Å c: 76.909Å
α: 90° β: 108.19° γ: 90°
R-values:
R R work R free
0.192 0.19 0.223
Expression systems:
  • Escherichia coli-Pichia pastoris shuttle vector pPpARG4
  • Not provided