› 5wx9

X-ray diffraction
1.76Å resolution

Crystal Structure of AtERF96 with GCC-box

Released:
DOI: 10.2210/pdb5wx9/pdb
PDB entry 5wx9 coloured by chain, front view.
PDB entry 5wx9 coloured by chain, side view.
PDB entry 5wx9 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box.
Chen CY, Lin PH, Chen KH, Cheng YS
Plant Mol Biol 104 483-498 (2020)
PMID: 32813232

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119367 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Ethylene-responsive transcription factor ERF096 Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.36 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9LSX0 (Residues: 1-131; Coverage: 100%)
Gene names: At5g43410, ERF096, MWF20.11
Sequence domains: AP2 domain
GCC-box motif Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.32 KDa
Source organism: Arabidopsis thaliana
Expression system: synthetic construct
GCC-box motif Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.39 KDa
Source organism: Arabidopsis thaliana
Expression system: synthetic construct

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P21
Unit cell:
a: 38.976Å b: 81.181Å c: 38.999Å
α: 90° β: 120.04° γ: 90°
R-values:
R R work R free
0.208 0.206 0.225
Expression systems:
  • Escherichia coli BL21(DE3)
  • synthetic construct

Quick links

Citations

1 review citation

Defense Strategies: The Role of Transcription Factors in Tomato-Pathogen Interaction.
Campos et al. (2022)
2 other articles

3 mentions without citation

Evolution analysis of FRIZZY PANICLE (FZP) orthologs explored the mutations in DNA coding sequences in the grass family (Poaceae).
Li et al. (2022)
2 more