› 5wxy

X-ray diffraction
2.63Å resolution

Crystal structure of Microcystis aeruginosa PCC 7806 aspartate racemase in complex with L-aspartate

Released:
DOI: 10.2210/pdb5wxy/pdb
PDB entry 5wxy coloured by chain, front view.
PDB entry 5wxy coloured by chain, side view.
PDB entry 5wxy coloured by chain, top view.
Source organism: Microcystis aeruginosa PCC 7806
Primary publication:
Structural insights into the catalysis and substrate specificity of cyanobacterial aspartate racemase McyF.
Cao DD, Zhang CP, Zhou K, Jiang YL, Tan XF, Xie J, Ren YM, Chen Y, Zhou CZ, Hou WT
Biochem Biophys Res Commun 514 1108-1114 (2019)
PMID: 31101340

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
McyF Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.21 KDa
Source organism: Microcystis aeruginosa PCC 7806
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RNB4 (Residues: 1-251; Coverage: 100%)
Gene names: IPF_371, mcyF
Sequence domains: Asp/Glu/Hydantoin racemase

Ligands and Environments

1 bound ligand:
Ligand ASP 1 x ASP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P3221
Unit cell:
a: 128.482Å b: 128.482Å c: 45.143Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.186 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Racemases and epimerases operating through a 1,1-proton transfer mechanism: reactivity, mechanism and inhibition.
Lloyd et al. (2021)
2 other articles