› 5x31

X-ray diffraction
2.6Å resolution

Pseudoazurin from Alcaligenes faecalis (space group P65)

Released:
DOI: 10.2210/pdb5x31/pdb
PDB entry 5x31 coloured by chain, front view.
PDB entry 5x31 coloured by chain, side view.
PDB entry 5x31 coloured by chain, top view.
Source organism: Alcaligenes faecalis
Primary publication:
New molecular packing in a crystal of pseudoazurin from Alcaligenes faecalis: a double-helical arrangement of blue copper.
Fukuda Y, Mizohata E, Inoue T
Acta Crystallogr F Struct Biol Commun 73 159-166 (2017)
PMID: 28291752

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137608 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pseudoazurin Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 13.67 KDa
Source organism: Alcaligenes faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04377 (Residues: 24-146; Coverage: 100%)
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 2 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P61
Unit cell:
a: 68.686Å b: 68.686Å c: 94.16Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

New molecular packing in a crystal of pseudoazurin from Alcaligenes faecalis: a double-helical arrangement of blue copper.
Fukuda et al. (2017)