› 5x3f

X-ray diffraction
3.38Å resolution

Crystal structure of the YgjG-Protein A-Zpa963-PKA catalytic domain

Released:
DOI: 10.2210/pdb5x3f/pdb
Model geometry
Fit model/data
PDB entry 5x3f coloured by chain, front view.
PDB entry 5x3f coloured by chain, side view.
PDB entry 5x3f coloured by chain, top view.
Source organisms:
Primary publication:
Construction of novel repeat proteins with rigid and predictable structures using a shared helix method.
Youn SJ, Kwon NY, Lee JH, Kim JH, Choi J, Lee H, Lee JO
OpenAccess logo Sci Rep 7 2595 (2017)
PMID: 28572639

Function and Biology Details

Reactions catalysed: 
An alpha,omega-diamine + 2-oxoglutarate = an omega-aminoaldehyde + L-glutamate
Putrescine + 2-oxoglutarate = L-glutamate + 4-aminobutanal + H(2)O
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138553 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Immunoglobulin G-binding protein A; Putrescine aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 501 amino acids
Theoretical weight: 54.45 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P42588 (Residues: 7-453; Coverage: 97%)
  • Canonical: P38507 (Residues: 220-269; Coverage: 11%)
Gene names: JW5510, b3073, patA, spa, ygjG
Sequence domains:
cAMP-dependent protein kinase catalytic subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 393 amino acids
Theoretical weight: 45.48 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 5-351; Coverage: 98%)
  • Best match: P05132-2 (Residues: 2-343)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: I222
Unit cell:
a: 140.518Å b: 153.496Å c: 205.714Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.227
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Development of imaging scaffolds for cryo-electron microscopy.
Yeates et al. (2020)
11 other articles