› 5x3k

X-ray diffraction
2.5Å resolution

Kfla1895 D451A mutant in complex with isomaltose

Released:
DOI: 10.2210/pdb5x3k/pdb
PDB entry 5x3k coloured by chain, front view.
PDB entry 5x3k coloured by chain, side view.
PDB entry 5x3k coloured by chain, top view.
Source organism: Kribbella flavida DSM 17836
Entry authors: Tanaka Y, Chen M, Tagami T, Yao M, Kimura A

Function and Biology Details

Reaction catalysed: 
(1a) cyclobis-(1->6)-alpha-nigerosyl + H(2)O = alpha-isomaltosyl-(1->3)-isomaltose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112381 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
1,3-alpha-isomaltosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 743 amino acids
Theoretical weight: 82.32 KDa
Source organism: Kribbella flavida DSM 17836
Expression system: Escherichia coli
UniProt:
  • Canonical: D2PPM7 (Residues: 1-723; Coverage: 100%)
Gene name: Kfla_1895
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand SO4 10 x SO4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: R32
Unit cell:
a: 180.584Å b: 180.584Å c: 392.291Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.182 0.216
Expression system: Escherichia coli

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