› 5x3v

X-ray diffraction
2.85Å resolution

Structure of human SHMT2 protein mutant

Released:
DOI: 10.2210/pdb5x3v/pdb
PDB entry 5x3v coloured by chain, front view.
PDB entry 5x3v coloured by chain, side view.
PDB entry 5x3v coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Li J, Sheng J

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152739 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 496 amino acids
Theoretical weight: 55.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P34897 (Residues: 22-504; Coverage: 96%)
Gene name: SHMT2
Sequence domains: Serine hydroxymethyltransferase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: P6522
Unit cell:
a: 158.655Å b: 158.655Å c: 209.358Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.177 0.231
Expression system: Escherichia coli BL21

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