› 5x3x

X-ray diffraction
2.79Å resolution

2.8A resolution structure of a cobalt energy-coupling factor transporter-CbiMQO

Released:
DOI: 10.2210/pdb5x3x/pdb
PDB entry 5x3x coloured by chain, front view.
PDB entry 5x3x coloured by chain, side view.
PDB entry 5x3x coloured by chain, top view.
Source organism: Rhodobacter capsulatus
Primary publication:
Structure and mechanism of a group-I cobalt energy coupling factor transporter.
Bao Z, Qi X, Hong S, Xu K, He F, Zhang M, Chen J, Chao D, Zhao W, Li D, Wang J, Zhang P
Cell Res 27 675-687 (2017)
PMID: 28322252

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-112677 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cobalt import ATP-binding protein CbiO Chains: A, B, a, b
Molecule details ›
Chains: A, B, a, b
Length: 280 amino acids
Theoretical weight: 29.46 KDa
Source organism: Rhodobacter capsulatus
Expression system: Escherichia coli
UniProt:
  • Canonical: O68106 (Residues: 1-257, 258-280; Coverage: 100%)
Gene names: RCAP_rcc02034, cbiO, cbiO2
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Cobalt transport protein CbiM Chains: M, m
Molecule details ›
Chains: M, m
Length: 222 amino acids
Theoretical weight: 22.55 KDa
Source organism: Rhodobacter capsulatus
Expression system: Escherichia coli
UniProt:
  • Canonical: D5AUZ9 (Residues: 1-222; Coverage: 100%)
Gene names: RCAP_rcc02037, cbiM
Sequence domains: Cobalt uptake substrate-specific transmembrane region
Cobalt transport protein CbiQ Chains: Q, q
Molecule details ›
Chains: Q, q
Length: 244 amino acids
Theoretical weight: 25.89 KDa
Source organism: Rhodobacter capsulatus
Expression system: Escherichia coli
UniProt:
  • Canonical: D5AUZ7 (Residues: 1-244; Coverage: 100%)
Gene names: RCAP_rcc02035, cbiQ, cbiQ2
Sequence domains: Cobalt transport protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2221
Unit cell:
a: 63.676Å b: 220.564Å c: 301.277Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.249
Expression system: Escherichia coli

Quick links

Citations

4 review citations

ECF-Type ATP-Binding Cassette Transporters.
Rempel et al. (2019)
3 more

5 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
4 more