› 5x41

X-ray diffraction
3.47Å resolution

3.5A resolution structure of a cobalt energy-coupling factor transporter using LCP method-CbiMQO

Released:
DOI: 10.2210/pdb5x41/pdb
PDB entry 5x41 coloured by chain, front view.
PDB entry 5x41 coloured by chain, side view.
PDB entry 5x41 coloured by chain, top view.
Source organism: Rhodobacter capsulatus
Primary publication:
Structure and mechanism of a group-I cobalt energy coupling factor transporter.
Bao Z, Qi X, Hong S, Xu K, He F, Zhang M, Chen J, Chao D, Zhao W, Li D, Wang J, Zhang P
Cell Res 27 675-687 (2017)
PMID: 28322252

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-102595 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ABC transporter ATP-binding protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 280 amino acids
Theoretical weight: 29.46 KDa
Source organism: Rhodobacter capsulatus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1G7HZ57 (Residues: 1-280; Coverage: 100%)
Gene name: SAMN04244550_01599
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Cobalt transport protein CbiM Chains: E, M
Molecule details ›
Chains: E, M
Length: 222 amino acids
Theoretical weight: 22.55 KDa
Source organism: Rhodobacter capsulatus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0Q0R232 (Residues: 1-222; Coverage: 100%)
Gene names: SAMN04244550_01602, cbiM
Sequence domains: Cobalt uptake substrate-specific transmembrane region
Cobalt/nickel transport system permease protein Chains: F, Q
Molecule details ›
Chains: F, Q
Length: 256 amino acids
Theoretical weight: 27.23 KDa
Source organism: Rhodobacter capsulatus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1G7HYD7 (Residues: 1-244; Coverage: 100%)
Gene name: SAMN04244550_01600
Sequence domains: Cobalt transport protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 96.159Å b: 135.04Å c: 204.424Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.3 0.299 0.335
Expression system: Escherichia coli

Quick links

Citations

4 review citations

ECF-Type ATP-Binding Cassette Transporters.
Rempel et al. (2019)
3 more

5 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
4 more