› 5x5l

X-ray diffraction
2.75Å resolution

Crystal structure of response regulator AdeR DNA binding domain in complex with an intercistronic region

Released:
DOI: 10.2210/pdb5x5l/pdb
PDB entry 5x5l coloured by chain, front view.
PDB entry 5x5l coloured by chain, side view.
PDB entry 5x5l coloured by chain, top view.
Source organisms:
Primary publication:
Mechanistic insight into how multidrug resistant Acinetobacter baumannii response regulator AdeR recognizes an intercistronic region.
Wen Y, Ouyang Z, Yu Y, Zhou X, Pei Y, Devreese B, Higgins PG, Zheng F
Nucleic Acids Res 45 9773-9787 (2017)
PMID: 28934482

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119376 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
AdeR Chains: A, B, E, H, M, N
Molecule details ›
Chains: A, B, E, H, M, N
Length: 109 amino acids
Theoretical weight: 12.71 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli
UniProt:
  • Canonical: E1A0Z5 (Residues: 139-247; Coverage: 44%)
Gene name: adeR
Sequence domains: Transcriptional regulatory protein, C terminal
DNA (5'-D(P*TP*AP*AP*AP*GP*TP*GP*TP*GP*GP*AP*GP*TP*AP*AP*GP*TP*GP*TP*GP*GP*AP*GP*A)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 25 nucleotides
Theoretical weight: 7.89 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*TP*CP*TP*CP*CP*AP*CP*AP*CP*TP*TP*AP*CP*TP*CP*CP*AP*CP*AP*CP*TP*TP*TP*A)-3') Chains: D, G
Molecule details ›
Chains: D, G
Length: 25 nucleotides
Theoretical weight: 7.46 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 55.51Å b: 71.38Å c: 78.36Å
α: 101.96° β: 104.53° γ: 101.86°
R-values:
R R work R free
0.279 0.278 0.296
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 citation in other articles

Diversity of mutations in regulatory genes of resistance-nodulation-cell division efflux pumps in association with tigecycline resistance in Acinetobacter baumannii.
Gerson et al. (2018)
10 more

3 mentions without citation

Mechanistic insight into how multidrug resistant Acinetobacter baumannii response regulator AdeR recognizes an intercistronic region.
Wen et al. (2017)
2 more