› 5xaa

X-ray diffraction
3.2Å resolution

Complete structure factors and an atomic model of the calcium pump (SERCA1A) and associated phospholipids in the E2-ALF-(TG) crystals of P21212 symmetry

Released:
DOI: 10.2210/pdb5xaa/pdb
PDB entry 5xaa coloured by chain, front view.
PDB entry 5xaa coloured by chain, side view.
PDB entry 5xaa coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Protein-phospholipid interplay revealed with crystals of a calcium pump.
Norimatsu Y, Hasegawa K, Shimizu N, Toyoshima C
Nature 545 193-198 (2017)
PMID: 28467821

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand ALF 1 x ALF
Ligand TG1 1 x TG1
Ligand PCW 33 x PCW
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 120.9Å b: 87.7Å c: 140.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.26

Quick links

Citations

24 review citations

Understanding the diversity of membrane lipid composition.
Harayama et al. (2018)
23 more

1 mention without citation

Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.
Enkavi et al. (2019)