› 5xas

X-ray diffraction
3.47Å resolution

Structural insights into the elevator-like mechanism of the sodium/citrate symporter CitS

Released:
DOI: 10.2210/pdb5xas/pdb
PDB entry 5xas coloured by chain, front view.
PDB entry 5xas coloured by chain, side view.
PDB entry 5xas coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Structural insights into the elevator-like mechanism of the sodium/citrate symporter CitS.
Kim JW, Kim S, Kim S, Lee H, Lee JO, Jin MS
Sci Rep 7 2548 (2017)
PMID: 28566738

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152038 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrate-sodium symporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 438 amino acids
Theoretical weight: 46.67 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: P31602 (Residues: 13-446; Coverage: 97%)
Gene name: citS
Sequence domains: 2-hydroxycarboxylate transporter family

Ligands and Environments

3 bound ligands:
Ligand NA 2 x NA
Ligand FLC 2 x FLC
Ligand BOG 1 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P1
Unit cell:
a: 51.705Å b: 83.34Å c: 81.06Å
α: 97.14° β: 100.16° γ: 94.59°
R-values:
R R work R free
0.248 0.246 0.295
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure and Mechanism of the Divalent Anion/Na⁺ Symporter.
Lu M. (2019)
9 other articles

3 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
2 more