› 5xat

X-ray diffraction
3.76Å resolution

Structural insights into the elevator-like mechanism of the sodium/citrate symporter CitS

Released:
DOI: 10.2210/pdb5xat/pdb
PDB entry 5xat coloured by chain, front view.
PDB entry 5xat coloured by chain, side view.
PDB entry 5xat coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Structural insights into the elevator-like mechanism of the sodium/citrate symporter CitS.
Kim JW, Kim S, Kim S, Lee H, Lee JO, Jin MS
Sci Rep 7 2548 (2017)
PMID: 28566738

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152038 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrate-sodium symporter Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 438 amino acids
Theoretical weight: 46.67 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: P31602 (Residues: 13-446; Coverage: 97%)
Gene name: citS
Sequence domains: 2-hydroxycarboxylate transporter family

Ligands and Environments

3 bound ligands:
Ligand NA 3 x NA
Ligand FLC 3 x FLC
Ligand BOG 1 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P1
Unit cell:
a: 87.668Å b: 91.874Å c: 96.32Å
α: 86.1° β: 82.22° γ: 69.66°
R-values:
R R work R free
0.269 0.267 0.298
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure and Mechanism of the Divalent Anion/Na⁺ Symporter.
Lu M. (2019)
9 other articles

2 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
1 more