› 5xce

X-ray diffraction
1.86Å resolution

Crystal structure of Wild type Vps29 from Entamoeba histolytica

Released:
DOI: 10.2210/pdb5xce/pdb
Model geometry
Fit model/data
PDB entry 5xce coloured by chain, front view.
PDB entry 5xce coloured by chain, side view.
PDB entry 5xce coloured by chain, top view.
Source organism: Entamoeba histolytica
Primary publication:
Structural and thermodynamic characterization of metal binding in Vps29 from Entamoeba histolytica: implication in retromer function.
Srivastava VK, Yadav R, Watanabe N, Tomar P, Mukherjee M, Gourinath S, Nakada-Tsukui K, Nozaki T, Datta S
Mol Microbiol 106 562-581 (2017)
PMID: 28898487

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189634 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar protein sorting-associated protein 29 Chains: A, B
Molecule details ›
Chains: A, B
Length: 193 amino acids
Theoretical weight: 21.88 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BI08 (Residues: 1-185; Coverage: 100%)
Gene names: CL6EHI_025270, EhVPS29, vsp
Sequence domains: Calcineurin-like phosphoesterase superfamily domain
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 35.564Å b: 78.771Å c: 67.974Å
α: 90° β: 90.1° γ: 90°
R-values:
R R work R free
0.2 0.198 0.247
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Molecular interplays of the Entamoeba histolytica endosomal sorting complexes required for transport during phagocytosis.
Bañuelos et al. (2022)
2 other articles