› 5xey

X-ray diffraction
1.82Å resolution

Discovery and structural analysis of a phloretin hydrolase from the opportunistic pathogen Mycobacterium abscessus

Released:
DOI: 10.2210/pdb5xey/pdb
PDB entry 5xey coloured by chain, front view.
PDB entry 5xey coloured by chain, side view.
PDB entry 5xey coloured by chain, top view.
Source organism: Mycobacteroides abscessus ATCC 19977
Primary publication:
Discovery and structural analysis of a phloretin hydrolase from the opportunistic human pathogen Mycobacterium abscessus.
Han JT, Zhang SP, Jia WJ, Zhang Z, Wang Y, He YX
FEBS J 286 1959-1971 (2019)
PMID: 30784195

Function and Biology Details

Reaction catalysed: 
Phloretin + H(2)O = phloretate + phloroglucinol
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phloretin hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 279 amino acids
Theoretical weight: 31.84 KDa
Source organism: Mycobacteroides abscessus ATCC 19977
Expression system: Escherichia coli
UniProt:
  • Canonical: B1MK49 (Residues: 2-279; Coverage: 99%)
Gene name: MAB_4487c
Sequence domains: DAPG hydrolase PhiG domain

Ligands and Environments

1 bound ligand:
Ligand 83X 1 x 83X
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 75.195Å b: 65.091Å c: 136.861Å
α: 90° β: 105.74° γ: 90°
R-values:
R R work R free
0.211 0.208 0.256
Expression system: Escherichia coli

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Citations

4 citation in other articles

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McGivern et al. (2021)
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