› 5xez

X-ray diffraction
3Å resolution

Structure of the Full-length glucagon class B G protein-coupled receptor

Released:
DOI: 10.2210/pdb5xez/pdb
PDB entry 5xez coloured by chain, front view.
PDB entry 5xez coloured by chain, side view.
PDB entry 5xez coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the full-length glucagon class B G-protein-coupled receptor.
Zhang H, Qiao A, Yang D, Yang L, Dai A, de Graaf C, Reedtz-Runge S, Dharmarajan V, Zhang H, Han GW, Grant TD, Sierra RG, Weierstall U, Nelson G, Liu W, Wu Y, Ma L, Cai X, Lin G, Wu X, Geng Z, Dong Y, Song G, Griffin PR, Lau J, Cherezov V, Yang H, Hanson MA, Stevens RC, Zhao Q, Jiang H, Wang MW, Wu B
Nature 546 259-264 (2017)
PMID: 28514451

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208298 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Endolysin; Glucagon receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 574 amino acids
Theoretical weight: 65.78 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P47871 (Residues: 27-256, 260-432; Coverage: 89%)
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
Gene names: E, GCGR
Sequence domains:
Antibody, mAb1, heavy chain Chains: C, H
Molecule details ›
Chains: C, H
Length: 231 amino acids
Theoretical weight: 24.98 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Antibody, mAb1, light chain Chains: D, L
Molecule details ›
Chains: D, L
Length: 214 amino acids
Theoretical weight: 23.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 3 x NAG
Ligand 97V 2 x 97V
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: P21
Unit cell:
a: 72.61Å b: 245.33Å c: 96.15Å
α: 90° β: 90.01° γ: 90°
R-values:
R R work R free
0.211 0.21 0.243
Expression systems:
  • Spodoptera frugiperda
  • Homo sapiens

Quick links

Citations

19 review citations

Mechanisms of signalling and biased agonism in G protein-coupled receptors.
Wootten et al. (2018)
18 more

32 mentions without citation

GPCR drug discovery: integrating solution NMR data with crystal and cryo-EM structures.
Shimada et al. (2019)
31 more