› 5xf1

X-ray diffraction
3.19Å resolution

Structure of the Full-length glucagon class B G protein-coupled receptor

Released:
DOI: 10.2210/pdb5xf1/pdb
PDB entry 5xf1 coloured by chain, front view.
PDB entry 5xf1 coloured by chain, side view.
PDB entry 5xf1 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the full-length glucagon class B G-protein-coupled receptor.
Zhang H, Qiao A, Yang D, Yang L, Dai A, de Graaf C, Reedtz-Runge S, Dharmarajan V, Zhang H, Han GW, Grant TD, Sierra RG, Weierstall U, Nelson G, Liu W, Wu Y, Ma L, Cai X, Lin G, Wu X, Geng Z, Dong Y, Song G, Griffin PR, Lau J, Cherezov V, Yang H, Hanson MA, Stevens RC, Zhao Q, Jiang H, Wang MW, Wu B
Nature 546 259-264 (2017)
PMID: 28514451

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208299 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Endolysin; Glucagon receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 574 amino acids
Theoretical weight: 65.78 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P47871 (Residues: 27-256, 260-432; Coverage: 89%)
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
Gene names: E, GCGR
Sequence domains:
Antibody mAb1 Heavy chain Chains: C, H
Molecule details ›
Chains: C, H
Length: 231 amino acids
Theoretical weight: 24.98 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Antibody mAb1 Light chain Chains: D, L
Molecule details ›
Chains: D, L
Length: 214 amino acids
Theoretical weight: 23.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand 97V 2 x 97V
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 71.46Å b: 248.79Å c: 93.33Å
α: 90° β: 90.16° γ: 90°
R-values:
R R work R free
0.207 0.205 0.231
Expression systems:
  • Spodoptera frugiperda
  • Homo sapiens

Quick links

Citations

24 review citations

Mechanisms of signalling and biased agonism in G protein-coupled receptors.
Wootten et al. (2018)
23 more

14 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
13 more