› 5xj1

X-ray diffraction
1.75Å resolution

Crystal structure of spRlmCD

Released:
DOI: 10.2210/pdb5xj1/pdb
PDB entry 5xj1 coloured by chain, front view.
PDB entry 5xj1 coloured by chain, side view.
PDB entry 5xj1 coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Structural insights into substrate selectivity of ribosomal RNA methyltransferase RlmCD.
Jiang Y, Li F, Wu J, Shi Y, Gong Q
PLoS One 12 e0185226 (2017)
PMID: 28949991

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized RNA methyltransferase SP_1029 Chain: A
Molecule details ›
Chain: A
Length: 454 amino acids
Theoretical weight: 51.39 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q97R12 (Residues: 1-454; Coverage: 84%)
Gene name: SP_1029
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand P6G 1 x P6G
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21
Unit cell:
a: 46.452Å b: 101.772Å c: 63.037Å
α: 90° β: 110.11° γ: 90°
R-values:
R R work R free
0.176 0.175 0.202
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 citation in other articles

Mouse Trmt2B protein is a dual specific mitochondrial metyltransferase responsible for m5U formation in both tRNA and rRNA.
Laptev et al. (2020)
1 more

2 mentions without citation

Structural insights into substrate selectivity of ribosomal RNA methyltransferase RlmCD.
Jiang et al. (2017)
1 more