› 5xj8

X-ray diffraction
2.41Å resolution

Crystal structure of PlsY (YgiH), an integral membrane glycerol 3-phosphate acyltransferase - the lysphosphatidic acid form

Released:
DOI: 10.2210/pdb5xj8/pdb
PDB entry 5xj8 coloured by chain, front view.
PDB entry 5xj8 coloured by chain, side view.
PDB entry 5xj8 coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Structural insights into the committed step of bacterial phospholipid biosynthesis.
Li Z, Tang Y, Wu Y, Zhao S, Bao J, Luo Y, Li D
Nat Commun 8 1691 (2017)
PMID: 29167463

Function and Biology Details

Reaction catalysed: 
An acyl-phosphate + sn-glycerol 3-phosphate = a 1-acyl-sn-glycerol 3-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol-3-phosphate acyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 201 amino acids
Theoretical weight: 21.89 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66905 (Residues: 3-192; Coverage: 99%)
Gene names: aq_676, plsY
Sequence domains: Glycerol-3-phosphate acyltransferase

Ligands and Environments

2 bound ligands:
Ligand PO4 3 x PO4
Ligand NKO 1 x NKO
1 modified residue:
Ligand FME 2 x FME

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 123.888Å b: 40.895Å c: 91.705Å
α: 90° β: 95.59° γ: 90°
R-values:
R R work R free
0.23 0.228 0.264
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Chemical tools for membrane protein structural biology.
Zhang et al. (2019)
2 more

1 mention without citation

Structural insights into the committed step of bacterial phospholipid biosynthesis.
Li et al. (2017)