› 5xkj

X-ray diffraction
3.48Å resolution

Crystal structure of plant receptor ERL1-TMM in complexe with EPF2

Released:
DOI: 10.2210/pdb5xkj/pdb
PDB entry 5xkj coloured by chain, front view.
PDB entry 5xkj coloured by chain, side view.
PDB entry 5xkj coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
A receptor-like protein acts as a specificity switch for the regulation of stomatal development.
Lin G, Zhang L, Han Z, Yang X, Liu W, Li E, Chang J, Qi Y, Shpak ED, Chai J
Genes Dev 31 927-938 (2017)
PMID: 28536146

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-111006 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein TOO MANY MOUTHS Chains: C, D
Molecule details ›
Chains: C, D
Length: 433 amino acids
Theoretical weight: 47.47 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9SSD1 (Residues: 28-460; Coverage: 92%)
Gene names: At1g80080, F18B13.16, RLP17, TMM
Sequence domains:
MEPF2 Chains: E, F
Molecule details ›
Chains: E, F
Length: 52 amino acids
Theoretical weight: 5.67 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q8LC53 (Residues: 69-120; Coverage: 55%)
Gene names: At1g34245, EPF2, F23M19
Sequence domains: Epidermal patterning factor proteins
LRR receptor-like serine/threonine-protein kinase ERL1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 555 amino acids
Theoretical weight: 60.42 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: C0LGW6 (Residues: 24-572; Coverage: 58%)
Gene names: At5g62230, ERL1, MMI9.14
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17B1
Spacegroup: P1
Unit cell:
a: 66.154Å b: 65.601Å c: 142.496Å
α: 102.86° β: 97.6° γ: 93.58°
R-values:
R R work R free
0.256 0.254 0.277
Expression system: Trichoplusia ni

Quick links

Citations

27 review citations

Plant cell surface receptor-mediated signaling - a common theme amid diversity.
He et al. (2018)
26 more