› 5xku

X-ray diffraction
1.78Å resolution

Crystal structure of hemagglutinin globular head from an H7N9 influenza virus in complex with a neutralizing antibody HNIgGA6

Released:
DOI: 10.2210/pdb5xku/pdb
PDB entry 5xku coloured by chain, front view.
PDB entry 5xku coloured by chain, side view.
PDB entry 5xku coloured by chain, top view.
Source organisms:
Primary publication:
Structural Insight into a Human Neutralizing Antibody against Influenza Virus H7N9.
Chen C, Liu L, Xiao Y, Cui S, Wang J, Jin Q
J Virol 92 (2018)
PMID: 29212936

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 35.95 KDa
Source organism: Influenza A virus
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: R4NN21 (Residues: 19-339; Coverage: 57%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin (Ha1 Chain); Chain: A; domain 1
HNIgGA6 light chain Chain: B
Molecule details ›
Chain: B
Length: 219 amino acids
Theoretical weight: 23.7 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector pCBio
Structure domains: Immunoglobulins
HNIgGA6 heavy chain Chain: C
Molecule details ›
Chain: C
Length: 233 amino acids
Theoretical weight: 24.68 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector pCBio
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P41212
Unit cell:
a: 70.983Å b: 70.983Å c: 283.728Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.242
Expression systems:
  • Insect cell expression vector pTIE1
  • Mammalian expression vector pCBio

Quick links

Citations

2 review citations

Clinical Advances in Viral-Vectored Influenza Vaccines.
Sebastian et al. (2018)
1 more

3 mentions without citation

Structural Insight into a Human Neutralizing Antibody against Influenza Virus H7N9.
Chen et al. (2018)
2 more