› 5xm2

X-ray diffraction
2.19Å resolution

Human N-terminal domain of FACT complex subunit SPT16

Released:
DOI: 10.2210/pdb5xm2/pdb
PDB entry 5xm2 coloured by chain, front view.
PDB entry 5xm2 coloured by chain, side view.
PDB entry 5xm2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structural basis of human Spt16 N-terminal domain interaction with histone (H3-H4)2 tetramer.
Jiang H, Xu S, Chen Y, Li H, Tian L, Zhou H, Zhao Z, Yang C, Zhong Z, Cai G, Su D
Biochem Biophys Res Commun 508 864-870 (2019)
PMID: 30528735

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195268 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FACT complex subunit SPT16 Chains: A, B
Molecule details ›
Chains: A, B
Length: 437 amino acids
Theoretical weight: 49.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y5B9 (Residues: 1-437; Coverage: 42%)
Gene names: FACT140, FACTP140, SUPT16H
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: F23
Unit cell:
a: 247.983Å b: 247.983Å c: 247.983Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.166 0.216
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Regulation of chromatin structure and function: insights into the histone chaperone FACT.
Wang et al. (2021)
2 other articles

2 mentions without citation

Structure-function relationship of H2A-H2B specific plant histone chaperones.
Kumar et al. (2020)
1 more