› 5xm6

X-ray diffraction
2.5Å resolution

the overall structure of VrEH2

Released:
DOI: 10.2210/pdb5xm6/pdb
PDB entry 5xm6 coloured by chain, front view.
PDB entry 5xm6 coloured by chain, side view.
PDB entry 5xm6 coloured by chain, top view.
Source organism: Vigna radiata
Primary publication:
Regioselectivity Engineering of Epoxide Hydrolase: Near-Perfect Enantioconvergence through a Single Site Mutation
Li FL, Kong XD, Chen Q, Zheng YC, Xu Q, Chen FF, Fan LQ, Lin GQ, Zhou JH, Yu HL, Xu JH
ACS Catalysis 8 8314-8317 (2018)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102656 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 352 amino acids
Theoretical weight: 39.82 KDa
Source organism: Vigna radiata
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0R5NGA4 (Residues: 1-318; Coverage: 100%)
Gene name: EH2
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P41212
Unit cell:
a: 109.18Å b: 109.18Å c: 236.967Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.223
Expression system: Escherichia coli BL21(DE3)

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