5xmk

Electron Microscopy
4.18Å resolution

Cryo-EM structure of the ATP-bound Vps4 mutant-E233Q complex with Vta1 (masked)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-156670 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vacuolar protein sorting-associated protein 4 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 437 amino acids
Theoretical weight: 48.23 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P52917 (Residues: 1-437; Coverage: 100%)
Gene names: CSC1, DID6, END13, GRD13, P9705.10, VPL4, VPS4, VPT10, YPR173C
Sequence domains:
Vacuolar protein sorting-associated protein VTA1 Chains: G, H, I, J, K, L, M, N
Molecule details ›
Chains: G, H, I, J, K, L, M, N
Length: 330 amino acids
Theoretical weight: 37.36 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06263 (Residues: 1-330; Coverage: 100%)
Gene names: VTA1, YLR181C
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.18Å
Relevant EMDB volumes: EMD-6734
Expression system: Escherichia coli