› 5xpx

X-ray diffraction
2.77Å resolution

Structure elucidation of truncated AMS3 lipase from an Antarctic Pseudomonas

Released:
DOI: 10.2210/pdb5xpx/pdb
PDB entry 5xpx coloured by chain, front view.
PDB entry 5xpx coloured by chain, side view.
PDB entry 5xpx coloured by chain, top view.
Source organism: Pseudomonas sp. A3(2015c)
Entry authors: Rahman RNZRA, Latip W

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
triacylglycerol lipase Chains: A, E
Molecule details ›
Chains: A, E
Length: 429 amino acids
Theoretical weight: 47.96 KDa
Source organism: Pseudomonas sp. A3(2015c)
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A0K0PTR1 (Residues: 1-388; Coverage: 100%)

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 86.753Å b: 94.693Å c: 126.431Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.277
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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