› 5xtr

X-ray diffraction
2.25Å resolution

Crystal structure of baculoviral sulfhydryl oxidase P33 (R127A, E183A mutant)

Released:
DOI: 10.2210/pdb5xtr/pdb
Model geometry
Fit model/data
PDB entry 5xtr coloured by chain, front view.
PDB entry 5xtr coloured by chain, side view.
PDB entry 5xtr coloured by chain, top view.
Source organism: Autographa californica nucleopolyhedrovirus
Primary publication:
Three Conserved Regions in Baculovirus Sulfhydryl Oxidase P33 Are Critical for Enzymatic Activity and Function.
Kuang W, Zhang H, Wang M, Zhou NY, Deng F, Wang H, Gong P, Hu Z
J Virol 91 (2017)
PMID: 28904203

Function and Biology Details

Reaction catalysed: 
2 R'C(R)SH + O(2) = R'C(R)S-S(R)CR' + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-linked sulfhydryl oxidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 293 amino acids
Theoretical weight: 34.38 KDa
Source organism: Autographa californica nucleopolyhedrovirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41480 (Residues: 1-259; Coverage: 100%)
Gene names: ORF92, P33
Sequence domains: Baculovirus P33

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P1
Unit cell:
a: 59.136Å b: 76.204Å c: 80.231Å
α: 84.32° β: 70.83° γ: 69.27°
R-values:
R R work R free
0.245 0.243 0.287
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 citation in other articles

Baculovirus Per Os Infectivity Factor Complex: Components and Assembly.
Wang et al. (2019)
7 more

1 mention without citation

Three Conserved Regions in Baculovirus Sulfhydryl Oxidase P33 Are Critical for Enzymatic Activity and Function.
Kuang et al. (2017)