› 5xv7

X-ray diffraction
2.32Å resolution

SRPK1 in complex with Alectinib

Released:
DOI: 10.2210/pdb5xv7/pdb
PDB entry 5xv7 coloured by chain, front view.
PDB entry 5xv7 coloured by chain, side view.
PDB entry 5xv7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
SRPKIN-1: A Covalent SRPK1/2 Inhibitor that Potently Converts VEGF from Pro-angiogenic to Anti-angiogenic Isoform.
Hatcher JM, Wu G, Zeng C, Zhu J, Meng F, Patel S, Wang W, Ficarro SB, Leggett AL, Powell CE, Marto JA, Zhang K, Ki Ngo JC, Fu XD, Zhang T, Gray NS
Cell Chem Biol 25 460-470.e6 (2018)
PMID: 29478907

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188751 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SRSF protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 372 amino acids
Theoretical weight: 42.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q96SB4 (Residues: 67-655; Coverage: 57%)
Gene name: SRPK1
Sequence domains: Protein kinase domain
Structure domains: Phosphorylase Kinase; domain 1

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand EMH 1 x EMH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P6522
Unit cell:
a: 74.767Å b: 74.767Å c: 310.687Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.212 0.238
Expression system: Escherichia coli K-12

Quick links

Citations

25 review citations

Roles and mechanisms of alternative splicing in cancer - implications for care.
Bonnal et al. (2020)
24 more

2 mentions without citation

Structure-based design and analysis of SuFEx chemical probes.
Jones et al. (2020)
1 more