› 5xwz

X-ray diffraction
1.75Å resolution

Crystal structure of a lactonase from Cladophialophora bantiana

Released:
DOI: 10.2210/pdb5xwz/pdb
PDB entry 5xwz coloured by chain, front view.
PDB entry 5xwz coloured by chain, side view.
PDB entry 5xwz coloured by chain, top view.
Source organism: Cladophialophora bantiana CBS 173.52
Primary publication:
Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana.
Hui R, Hu X, Liu W, Liu W, Zheng Y, Chen Y, Guo RT, Jin J, Chen CC
Acta Crystallogr F Struct Biol Commun 73 515-519 (2017)
PMID: 28876230

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 271 amino acids
Theoretical weight: 29.93 KDa
Source organism: Cladophialophora bantiana CBS 173.52
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0D2H023 (Residues: 1-265; Coverage: 100%)
Gene name: Z519_12792
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

3 bound ligands:
Ligand NA 4 x NA
Ligand MLI 2 x MLI
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P21212
Unit cell:
a: 120.863Å b: 104.399Å c: 116.834Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.181
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Enzymes for Detoxification of Various Mycotoxins: Origins and Mechanisms of Catalytic Action.
Lyagin et al. (2019)
3 more

1 mention without citation

Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana.
Hui et al. (2017)