› 5xz8

X-ray diffraction
1.95Å resolution

Crystal Structure of Phosphofructokinase from Staphylococcus aureus in complex with adenylylimidodiphosphate (the ATP analog) and fructose-6-phosphate

Released:
DOI: 10.2210/pdb5xz8/pdb
PDB entry 5xz8 coloured by chain, front view.
PDB entry 5xz8 coloured by chain, side view.
PDB entry 5xz8 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
Primary publication:
Structural Insights into the Regulation of Staphylococcus aureus Phosphofructokinase by Tetramer-Dimer Conversion.
Tian T, Wang C, Wu M, Zhang X, Zang J
Biochemistry 57 4252-4262 (2018)
PMID: 29940104

Function and Biology Details

Reaction catalysed: 
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173455 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 35.95 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2FXM8 (Residues: 1-322; Coverage: 100%)
Gene names: SAOUHSC_01807, pfkA
Sequence domains: Phosphofructokinase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand F6P 1 x F6P
Ligand GOL 3 x GOL
Ligand MG 1 x MG
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: I222
Unit cell:
a: 75.36Å b: 79.83Å c: 120.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.21 0.262
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Identification of Alternative Allosteric Sites in Glycolytic Enzymes for Potential Use as Species-Specific Drug Targets.
Ayyildiz et al. (2020)
2 more