› 5y17

X-ray diffraction
2.3Å resolution

CATPO mutant - E316F

Released:
DOI: 10.2210/pdb5y17/pdb
PDB entry 5y17 coloured by chain, front view.
PDB entry 5y17 coloured by chain, side view.
PDB entry 5y17 coloured by chain, top view.
Source organism: Mycothermus thermophilus
Primary publication:
Identification of the site of oxidase substrate binding in Scytalidium thermophilum catalase.
Yuzugullu Karakus Y, Goc G, Balci S, Yorke BA, Trinh CH, McPherson MJ, Pearson AR
Acta Crystallogr D Struct Biol 74 979-985 (2018)
PMID: 30289408

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125992 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 678 amino acids
Theoretical weight: 74.77 KDa
Source organism: Mycothermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: M4GGR7 (Residues: 22-699; Coverage: 97%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HDD 4 x HDD
1 bound ligand:
Ligand CA 10 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Unit cell:
a: 125.733Å b: 120.87Å c: 183.804Å
α: 90° β: 101.97° γ: 90°
R-values:
R R work R free
0.18 0.178 0.22
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Monofunctional Heme-Catalases.
Hansberg W. (2022)
1 other article

1 mention without citation

Identification of the site of oxidase substrate binding in Scytalidium thermophilum catalase.
Yuzugullu Karakus et al. (2018)