› 5y23

X-ray diffraction
1.4Å resolution

X-ray crystal structure of Pseudoazurin Met16Phe variant

Released:
DOI: 10.2210/pdb5y23/pdb
PDB entry 5y23 coloured by chain, front view.
PDB entry 5y23 coloured by chain, side view.
PDB entry 5y23 coloured by chain, top view.
Source organism: Achromobacter cycloclastes
Entry authors: Yamaguchi T, Akao K, Kohzuma T

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148646 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pseudoazurin Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.05 KDa
Source organism: Achromobacter cycloclastes
Expression system: Escherichia coli
UniProt:
  • Canonical: P19567 (Residues: 29-152; Coverage: 100%)
Gene name: bcp
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 2 x CU
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 34.715Å b: 59.62Å c: 54.324Å
α: 90° β: 105.37° γ: 90°
R-values:
R R work R free
0.143 0.141 0.182
Expression system: Escherichia coli

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