› 5y2p

X-ray diffraction
1.5Å resolution

Crystal Structure of Bacillus sp. TB-90 Urate Oxidase Improved by Humidity Control at 89% RH

Released:
DOI: 10.2210/pdb5y2p/pdb
Model geometry
Fit model/data
PDB entry 5y2p coloured by chain, front view.
PDB entry 5y2p coloured by chain, side view.
PDB entry 5y2p coloured by chain, top view.
Source organism: Bacillus sp. TB-90
Entry authors: Itoh T, Nishiya Y

Function and Biology Details

Reactions catalysed: 
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175071 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uric acid degradation bifunctional protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 35.71 KDa
Source organism: Bacillus sp. TB-90
Expression system: Escherichia coli
UniProt:
  • Canonical: Q45697 (Residues: 178-489; Coverage: 62%)
Gene name: uao
Sequence domains: Uricase
Structure domains: Urate Oxidase;

Ligands and Environments

5 bound ligands:
Ligand AZA 2 x AZA
Ligand OXY 2 x OXY
Ligand SO4 1 x SO4
Ligand EDO 6 x EDO
Ligand K 1 x K
1 modified residue:
Ligand OCS 2 x OCS

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: I222
Unit cell:
a: 71.095Å b: 133.885Å c: 145.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.177
Expression system: Escherichia coli

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