› 5y58

X-ray diffraction
2.8Å resolution

Crystal structure of Ku70/80 and TLC1

Released:
DOI: 10.2210/pdb5y58/pdb
PDB entry 5y58 coloured by chain, front view.
PDB entry 5y58 coloured by chain, side view.
PDB entry 5y58 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural Insights into Yeast Telomerase Recruitment to Telomeres.
Chen H, Xue J, Churikov D, Hass EP, Shi S, Lemon LD, Luciano P, Bertuch AA, Zappulla DC, Géli V, Wu J, Lei M
Cell 172 331-343.e13 (2018)
PMID: 29290466

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152465 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
ATP-dependent DNA helicase II subunit 1 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 575 amino acids
Theoretical weight: 67.71 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P32807 (Residues: 28-602; Coverage: 96%)
Gene names: HDF1, NES24, YKU70, YM8021.10, YMR284W
Sequence domains:
ATP-dependent DNA helicase II subunit 2 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 628 amino acids
Theoretical weight: 71.19 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q04437 (Residues: 2-629; Coverage: 100%)
Gene names: HDF2, YKU80, YM9718.05C, YMR106C
Sequence domains:
TLC1 Chains: X, Y, Z
Molecule details ›
Chains: X, Y, Z
Length: 30 nucleotides
Theoretical weight: 9.64 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1, SSRF BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 116.296Å b: 115.159Å c: 115.847Å
α: 77.43° β: 78.48° γ: 63.73°
R-values:
R R work R free
0.218 0.215 0.258
Expression system: Spodoptera frugiperda

Quick links

Citations

15 review citations

Long non-coding RNAs: definitions, functions, challenges and recommendations.
Mattick et al. (2023)
14 more

7 mentions without citation

Telomerase structures and regulation: shedding light on the chromosome end.
Nguyen et al. (2019)
6 more