› 5y5d

X-ray diffraction
1.85Å resolution

The crystal structure of VrEH2 mutant M263W

Released:
DOI: 10.2210/pdb5y5d/pdb
PDB entry 5y5d coloured by chain, front view.
PDB entry 5y5d coloured by chain, side view.
PDB entry 5y5d coloured by chain, top view.
Source organism: Vigna radiata
Entry authors: Xu JH, Yu HL, Zhou JH, Kong XD, Li FL

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 326 amino acids
Theoretical weight: 37.39 KDa
Source organism: Vigna radiata
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0R5NGA4 (Residues: 1-318; Coverage: 100%)
Gene name: EH2
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P43212
Unit cell:
a: 70.475Å b: 70.475Å c: 129.021Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.236
Expression system: Escherichia coli BL21(DE3)

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