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› 5y5f

X-ray diffraction
1.5Å resolution

Function and Biology Details

Reaction catalysed: 
(1) nitrous oxide + NAD(+) + H2O = 2 nitric oxide + NADH + H(+).
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149924 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP nitrous oxide-forming nitric oxide reductase Chain: A
Molecule details ›
Chain: A
Length: 403 amino acids
Theoretical weight: 44.42 KDa
Source organism: Fusarium oxysporum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23295 (Residues: 1-403; Coverage: 100%)
Gene names: CYP55, CYP55A1
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand NO 1 x NO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 54.916Å b: 75.667Å c: 101.811Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Role of Computational Methods in Going beyond X-ray Crystallography to Explore Protein Structure and Dynamics.
Srivastava et al. (2018)
10 more

2 mentions without citation

Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate.
Tosha et al. (2017)
1 more