› 5y6j

X-ray diffraction
2.81Å resolution

Structure of Tomato spotted wilt virus nucleocapsid protein with alternative oligomerization state

Released:
DOI: 10.2210/pdb5y6j/pdb
Model geometry
Fit model/data
PDB entry 5y6j coloured by chain, front view.
PDB entry 5y6j coloured by chain, side view.
PDB entry 5y6j coloured by chain, top view.
Source organism: Orthotospovirus tomatomaculae
Primary publication:
Distinct Mechanism for the Formation of the Ribonucleoprotein Complex of Tomato Spotted Wilt Virus.
Guo Y, Liu B, Ding Z, Li G, Liu M, Zhu D, Sun Y, Dong S, Lou Z
J Virol 91 (2017)
PMID: 28904194

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124842 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoprotein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 258 amino acids
Theoretical weight: 28.84 KDa
Source organism: Orthotospovirus tomatomaculae
Expression system: Escherichia coli
UniProt:
  • Canonical: H6UMW9 (Residues: 1-258; Coverage: 100%)
Sequence domains: Tospovirus nucleocapsid protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 55.364Å b: 76.514Å c: 98.44Å
α: 90° β: 93.55° γ: 90°
R-values:
R R work R free
0.234 0.232 0.275
Expression system: Escherichia coli

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Citations

4 review citations

The RNA-Dependent RNA Polymerase NIb of Potyviruses Plays Multifunctional, Contrasting Roles during Viral Infection.
Shen et al. (2020)
3 more

3 mentions without citation

Distinct Mechanism for the Formation of the Ribonucleoprotein Complex of Tomato Spotted Wilt Virus.
Guo et al. (2017)
2 more