› 5ydj

X-ray diffraction
3.04Å resolution

Crystal structure of anopheles gambiae acetylcholinesterase in complex with PMSF

Released:
DOI: 10.2210/pdb5ydj/pdb
PDB entry 5ydj coloured by chain, front view.
PDB entry 5ydj coloured by chain, side view.
PDB entry 5ydj coloured by chain, top view.
Source organism: Anopheles gambiae
Entry authors: Han Q, Guan H, Ding H, Liao C, Robinson H, Li J

Function and Biology Details

Reaction catalysed: 
Acetylcholine + H(2)O = choline + acetate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Acetylcholinesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 737 amino acids
Theoretical weight: 81.14 KDa
Source organism: Anopheles gambiae
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q869C3 (Residues: 1-737; Coverage: 100%)
Gene names: ACE1, ACHE1, AGAP001356, Ace
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 2 x NAG
Ligand SO4 29 x SO4
Ligand CO 2 x CO
1 modified residue:
Ligand SEB 2 x SEB

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P61
Unit cell:
a: 147.65Å b: 147.65Å c: 224.18Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.253 0.251 0.286
Expression system: Komagataella pastoris

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