› 5yew

X-ray diffraction
3.2Å resolution

Structural basis for GTP hydrolysis and conformational change of mitofusin 1 in mediating mitochondrial fusion

Released:
DOI: 10.2210/pdb5yew/pdb
PDB entry 5yew coloured by chain, front view.
PDB entry 5yew coloured by chain, side view.
PDB entry 5yew coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for GTP hydrolysis and conformational change of MFN1 in mediating membrane fusion.
Yan L, Qi Y, Huang X, Yu C, Lan L, Guo X, Rao Z, Hu J, Lou Z
Nat Struct Mol Biol 25 233-243 (2018)
PMID: 29483649

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-184885 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitofusin-1 Chain: A
Molecule details ›
Chain: A
Length: 421 amino acids
Theoretical weight: 47.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IWA4 (Residues: 1-364, 374-741; Coverage: 56%)
Gene name: MFN1
Sequence domains: Dynamin family
Mitofusin-1 Chains: B, C
Molecule details ›
Chains: B, C
Length: 421 amino acids
Theoretical weight: 47.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IWA4 (Residues: 1-364, 374-741; Coverage: 56%)
Gene name: MFN1
Sequence domains: Dynamin family

Ligands and Environments

4 bound ligands:
Ligand GDP 3 x GDP
Ligand BEF 3 x BEF
Ligand K 3 x K
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P3221
Unit cell:
a: 207.942Å b: 207.942Å c: 107.893Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.223 0.257
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Mitochondrial dynamics and their potential as a therapeutic target.
Whitley et al. (2019)
23 more

11 mentions without citation

Recent insights into the structure and function of Mitofusins in mitochondrial fusion.
Cohen et al. (2018)
10 more