› 5yj3

X-ray diffraction
2.84Å resolution

Crystal structure of TZAP and telomeric DNA complex

Released:
DOI: 10.2210/pdb5yj3/pdb
PDB entry 5yj3 coloured by chain, front view.
PDB entry 5yj3 coloured by chain, side view.
PDB entry 5yj3 coloured by chain, top view.
Source organisms:
Primary publication:
The 11th C2H2 zinc finger and an adjacent C-terminal arm are responsible for TZAP recognition of telomeric DNA.
Zhao Y, Zhang G, He C, Mei Y, Shi Y, Li F
Cell Res 28 130-134 (2018)
PMID: 29134956

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119439 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Telomere zinc finger-associated protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 107 amino acids
Theoretical weight: 13.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10074 (Residues: 516-620; Coverage: 15%)
Gene names: HKR3, TZAP, ZBTB48, ZNF855
Sequence domains: Zinc finger, C2H2 type
DNA (5'-D(*CP*CP*TP*AP*AP*CP*CP*CP*TP*AP*AP*CP*CP*CP*TP*AP*AP*C)-3') Chain: A
Molecule details ›
Chain: A
Length: 18 nucleotides
Theoretical weight: 5.35 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 18 nucleotides
Theoretical weight: 5.68 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P43212
Unit cell:
a: 56.713Å b: 56.713Å c: 226.229Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.268
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

How stem cells keep telomeres in check.
Li et al. (2018)
1 more

1 mention without citation

The 11th C2H2 zinc finger and an adjacent C-terminal arm are responsible for TZAP recognition of telomeric DNA.
Zhao et al. (2018)