Function and Biology Details
Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
ORF1ab and ORF1a (preferred)
PDBe Complex ID:
PDB-CPX-125466 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ORF1ab
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 33.74 KDa
Source organism: Human betacoronavirus 2c EMC/2012
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Coronavirus 2'-O-methyltransferase
Structure domains: Vaccinia Virus protein VP39
Length: 303 amino acids
Theoretical weight: 33.74 KDa
Source organism: Human betacoronavirus 2c EMC/2012
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
K0BWD0 (Residues: 6776-7078; Coverage: 4%)
Sequence domains: Coronavirus 2'-O-methyltransferase
Structure domains: Vaccinia Virus protein VP39
ORF1a
Molecule details ›
Chain: B
Length: 140 amino acids
Theoretical weight: 14.9 KDa
Source organism: Human betacoronavirus 2c EMC/2012
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Coronavirus RNA synthesis protein NSP10
Length: 140 amino acids
Theoretical weight: 14.9 KDa
Source organism: Human betacoronavirus 2c EMC/2012
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: K4LC41 (Residues: 4238-4377; Coverage: 3%)
Sequence domains: Coronavirus RNA synthesis protein NSP10
