› 5yo3

X-ray diffraction
1.7Å resolution

Crystal Structure of B562RIL with engineered disulfide bond V16C-A29C

Released:
DOI: 10.2210/pdb5yo3/pdb
PDB entry 5yo3 coloured by chain, front view.
PDB entry 5yo3 coloured by chain, side view.
PDB entry 5yo3 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Protein crystal quality oriented disulfide bond engineering.
Pu M, Xu Z, Peng Y, Hou Y, Liu D, Wang Y, Liu H, Song G, Liu ZJ
Protein Cell 9 659-663 (2018)
PMID: 29039033

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-142149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 11.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2221
Unit cell:
a: 40.328Å b: 50.126Å c: 94.237Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.155 0.188
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Prediction of disulfide bond engineering sites using a machine learning method.
Gao et al. (2020)
5 more

1 mention without citation

Approximating lattice similarity.
Andrews et al. (2023)