› 5yqj

X-ray diffraction
1.5Å resolution

Crystal structure of the first StARkin domain of Lam4

Released:
DOI: 10.2210/pdb5yqj/pdb
PDB entry 5yqj coloured by chain, front view.
PDB entry 5yqj coloured by chain, side view.
PDB entry 5yqj coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis of sterol recognition and nonvesicular transport by lipid transfer proteins anchored at membrane contact sites.
Tong J, Manik MK, Im YJ
Proc Natl Acad Sci U S A 115 E856-E865 (2018)
PMID: 29339490

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153723 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane-anchored lipid-binding protein LAM4 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 187 amino acids
Theoretical weight: 20.53 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38800 (Residues: 749-929; Coverage: 14%)
Gene names: LAM4, LTC3, YHR080C
Sequence domains: VAD1 Analog of StAR-related lipid transfer domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21
Unit cell:
a: 38.813Å b: 54.95Å c: 119.478Å
α: 90° β: 95.08° γ: 90°
R-values:
R R work R free
0.16 0.158 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Here, there, and everywhere: The importance of ER membrane contact sites.
Wu et al. (2018)
14 more

3 mentions without citation

The History and Science of the Major Birch Pollen Allergen Bet v 1.
Breiteneder et al. (2023)
2 more