› 5yqq

X-ray diffraction
1.9Å resolution

Crystal structure of a domain-swapped dimer of the second StARkin domain of Lam2

Released:
DOI: 10.2210/pdb5yqq/pdb
PDB entry 5yqq coloured by chain, front view.
PDB entry 5yqq coloured by chain, side view.
PDB entry 5yqq coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis of sterol recognition and nonvesicular transport by lipid transfer proteins anchored at membrane contact sites.
Tong J, Manik MK, Im YJ
Proc Natl Acad Sci U S A 115 E856-E865 (2018)
PMID: 29339490

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane-anchored lipid-binding protein YSP2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.13 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06681 (Residues: 1060-1223; Coverage: 11%)
Gene names: LAM2, LTC4, YDR326C, YSP2
Sequence domains: VAD1 Analog of StAR-related lipid transfer domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P1
Unit cell:
a: 33.862Å b: 52.124Å c: 53.021Å
α: 107.81° β: 90.17° γ: 105.05°
R-values:
R R work R free
0.196 0.194 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Here, there, and everywhere: The importance of ER membrane contact sites.
Wu et al. (2018)
14 more

2 mentions without citation

The History and Science of the Major Birch Pollen Allergen Bet v 1.
Breiteneder et al. (2023)
1 more