› 5yqr

X-ray diffraction
2.4Å resolution

Crystal structure of the PH-like domain of Lam6

Released:
DOI: 10.2210/pdb5yqr/pdb
PDB entry 5yqr coloured by chain, front view.
PDB entry 5yqr coloured by chain, side view.
PDB entry 5yqr coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of sterol recognition and nonvesicular transport by lipid transfer proteins anchored at membrane contact sites.
Tong J, Manik MK, Im YJ
Proc Natl Acad Sci U S A 115 E856-E865 (2018)
PMID: 29339490

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133002 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Membrane-anchored lipid-binding protein LAM6 Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.73 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
  • Canonical: Q08001 (Residues: 161-272; Coverage: 16%)
Gene names: E, LAM6, LTC1, YLR072W
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand 2PE 1 x 2PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 59.543Å b: 60.103Å c: 105.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Here, there, and everywhere: The importance of ER membrane contact sites.
Wu et al. (2018)
15 more

3 mentions without citation

Structural basis of sterol recognition and nonvesicular transport by lipid transfer proteins anchored at membrane contact sites.
Tong et al. (2018)
2 more