› 5ys0

X-ray diffraction
2.6Å resolution

Crystal structure of the second StARkin domain of Lam2 in complex with ergosterol

Released:
DOI: 10.2210/pdb5ys0/pdb
PDB entry 5ys0 coloured by chain, front view.
PDB entry 5ys0 coloured by chain, side view.
PDB entry 5ys0 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis of sterol recognition and nonvesicular transport by lipid transfer proteins anchored at membrane contact sites.
Tong J, Manik MK, Im YJ
Proc Natl Acad Sci U S A 115 E856-E865 (2018)
PMID: 29339490

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170374 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane-anchored lipid-binding protein YSP2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 170 amino acids
Theoretical weight: 19.13 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06681 (Residues: 1060-1223; Coverage: 11%)
Gene names: LAM2, LTC4, YDR326C, YSP2
Sequence domains: VAD1 Analog of StAR-related lipid transfer domain

Ligands and Environments

1 bound ligand:
Ligand ERG 2 x ERG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: C2
Unit cell:
a: 189.717Å b: 70.315Å c: 42.508Å
α: 90° β: 103.14° γ: 90°
R-values:
R R work R free
0.244 0.241 0.294
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Here, there, and everywhere: The importance of ER membrane contact sites.
Wu et al. (2018)
15 more

2 mentions without citation

Structural basis of sterol recognition and nonvesicular transport by lipid transfer proteins anchored at membrane contact sites.
Tong et al. (2018)
1 more