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› 5ysw

X-ray diffraction
2.6Å resolution

Crystal Structure Analysis of Rif16 in complex with R-L

Released:
DOI: 10.2210/pdb5ysw/pdb
Model geometry
Fit model/data
PDB entry 5ysw coloured by chain, front view.
PDB entry 5ysw coloured by chain, side view.
PDB entry 5ysw coloured by chain, top view.
Source organism: Amycolatopsis mediterranei U32
Primary publication:
Deciphering the late steps of rifamycin biosynthesis.
Qi F, Lei C, Li F, Zhang X, Wang J, Zhang W, Fan Z, Li W, Tang GL, Xiao Y, Zhao G, Li S
OpenAccess logo Nat Commun 9 2342 (2018)
PMID: 29904078

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101849 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chain: A
Molecule details ›
Chain: A
Length: 441 amino acids
Theoretical weight: 48.79 KDa
Source organism: Amycolatopsis mediterranei U32
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3CVZ6 (Residues: 2-420; Coverage: 100%)
Gene name: AMED_0653
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand 9LF 1 x 9LF
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 35.082Å b: 70.709Å c: 80.975Å
α: 90° β: 94° γ: 90°
R-values:
R R work R free
0.208 0.205 0.271
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications.
Li et al. (2020)
3 more

1 mention without citation

Deciphering the late steps of rifamycin biosynthesis.
Qi et al. (2018)