› 5yvj

X-ray diffraction
2.51Å resolution

Crystal structure of full length NS2B47-NS3 (gD4NS2BNS3) from Dengue virus 4 in open conformation

Released:
DOI: 10.2210/pdb5yvj/pdb
PDB entry 5yvj coloured by chain, front view.
PDB entry 5yvj coloured by chain, side view.
PDB entry 5yvj coloured by chain, top view.
Source organism: dengue virus type 4
Entry authors: Phoo WW, El Sahili A

Function and Biology Details

Reactions catalysed: 
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123727 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Genome polyprotein Chain: B
Molecule details ›
Chain: B
Length: 618 amino acids
Theoretical weight: 69.5 KDa
Source organism: dengue virus type 4
Expression system: Escherichia coli
UniProt:
  • Canonical: F8TEL4 (Residues: 1475-2092; Coverage: 18%)
Sequence domains:
Genome polyprotein Chain: C
Molecule details ›
Chain: C
Length: 47 amino acids
Theoretical weight: 5.2 KDa
Source organism: dengue virus type 4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2YHE8 (Residues: 1393-1439; Coverage: 1%)
Sequence domains: Flavivirus non-structural protein NS2B

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 52.78Å b: 86.81Å c: 75.9Å
α: 90° β: 93.07° γ: 90°
R-values:
R R work R free
0.191 0.188 0.246
Expression system: Escherichia coli

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