› 5yx6

X-ray diffraction
2.2Å resolution

Crystal structure of Rv3272 from M. tuberculosis orthorhombic form

Released:
DOI: 10.2210/pdb5yx6/pdb
PDB entry 5yx6 coloured by chain, front view.
PDB entry 5yx6 coloured by chain, side view.
PDB entry 5yx6 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Rv3272 encodes a novel Family III CoA transferase that alters the cell wall lipid profile and protects mycobacteria from acidic and oxidative stress.
Karade SS, Pandey S, Ansari A, Das S, Tripathi S, Arora A, Chopra S, Pratap JV, Dasgupta A
Biochim Biophys Acta Proteins Proteom 1867 317-330 (2019)
PMID: 30342240

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161257 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable fatty acyl-CoA transferase Rv3272 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 394 amino acids
Theoretical weight: 42.51 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P96877 (Residues: 1-394; Coverage: 100%)
Gene name: Rv3272
Sequence domains: CoA-transferase family III

Ligands and Environments

2 bound ligands:
Ligand BEN 7 x BEN
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P212121
Unit cell:
a: 68.261Å b: 101.922Å c: 241.324Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Crystal Structure of an Intramolecular Mesaconyl-Coenzyme A Transferase From the 3-Hydroxypropionic Acid Cycle of Roseiflexus castenholzii.
Min et al. (2022)
1 more